葡萄芪合酶基因家族上游调控序列分析

文章编号：2095-1191（2016）01-0001-06

Analysis on upstream regulatory sequence of grape stilbene synthase gene family

WANG Chao-xia， ZHANG Ya-li， LU Jiang *

（College of Food Science and Nutritional Engineering， China Agricultural University， Beijing 10083， China）

Abstract：【Objective】In order to provide theoretical basis for studying effect of stilbene synthase（STS） gene on grape growth and stress-response mechanisms， the present experiment aimed to investigate the cis-acting regulation elements in promoter region of STS genes and upstream regulatory sequences of STS genes from different organisms. 【Method】Based on genome sequence of Vitis vinifera‘PN40024’， 48 STS family genes was predicted. In addition， the reported STS genes from 8 grape cultivars viz.， Muscadine rotundifolia ‘Noble’， Vitis pseudoreticulata ‘Baihe 35-1’ and V. vinifera ‘Thompson Seedless’， ‘Red Globe’ and ‘Cabernet Sauvignon’ were also selected. Then， the cis-acting elements in upstream regulatory sequences of those STS genes were analyzed using PlantCARE database. 【Result】The result showed that， there existed many cis-acting elements in upstream regulatory sequences of STS genes， which could be classified into 8 categories viz.， component specific， elicitor specific， binding site specific， condition specific， plant tissues specific， regulation specific， light responsiveness and transcription elements. Of which， some elements such as TCA-element， ABRE， ERF， P-box， Box-W1 and TC-rich repeats were related to grape resistance. Furthermore， there were differences in the number of cis-acting elements in upstream regulatory sequences between these STS genes， as follows： the upstream regulatory sequence of V. vinifera ‘Carinena’ VvPinSTS26 gene had the most number of cis-acting elements（49）， while VvPinSTS24 gene had the fewest number of cis-acting elements（only 2 ones）. 【Conclusion】Based on above-mentioned results， it can be inferred that the STS gene expression is affected by ABA， SA， GA， ethylene and fungal elicitor， so that these elicitors play a role in regulating resveratrol synthesis in the grape defense process.